技术文章/ARTICLE
生克利好斗受要见指四放海后想就老知由江(暴雨/天气)
点击次数：2989 发布时间：2022/9/15 14:55:03
检测原理方正姚体试剂盒采用双抗体夹心法酶联免疫吸附试验。往预先包被人乳酸脱氢酶（方正姚体）捕获抗体的包被微孔中，依次加入标本、标准品、方正姚体_utf-8">HRP标记的检测抗体，经过温育并彻di洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成终的黄色。颜色的深浅和样品中的人乳酸脱氢酶（LDH方正姚体_）呈正相关。用酶标仪在方正姚体_450nm 波长下测定吸方正姚体_光度（方正姚体_OD 值），计算样品浓度。样品收集、处理及保存方法1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。
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